Gatland’s side face an Italian outfit that were beaten 31-19 by Scotland in Edinburgh in the opening round of matches.
Wales have not been beaten in Rome since 2007 and their last Six Nations victory was the away trip to Italy two years ago.
This has been followed by seven consecutive defeats in the tournament and defeat this week will leave Gatland’s squad contemplating consecutive Wooden Spoons with home matches against Ireland and England and a trip to Scotland to come.
Another loss will also probably see Wales drop to a lowest position of 12th in the world rankings.
“This has been an important week with a lot of hard work put in during training,” said Gatland.
“We want to be accurate and disciplined on Saturday. It’s about our execution and how we manage the game.
“We know Italy are a quality side with physical players and are looking forward to a good contest. We’re excited for the challenge on Saturday.”
Wales: L Williams; Rogers, Tompkins, James, Adams; B Thomas, T Williams; G Thomas, Lloyd, H Thomas, Rowlands, Jenkins, Botham, Morgan (capt), Faletau.
Replacements: Dee, Smith, Assiratti, F Thomas, Wainwright, R Williams, Edwards, Murray.
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